Quality Control

Trityl Monitoring of Coupling Efficiency

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As an initial quality control procedure, MIDLAND verifies the coupling efficiency during the synthesis process by monitoring the intensity of the dimethoxytrityl cation during the acid-induced cleavage process. Failure of a sequence to meet our minimum requirements results in the automatic resynthesis of the sequence.

Yield From Synthesis

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Yield of a particular synthesis is affected by a large number of factors, including:

  • Scale of synthesis
  • Base Composition of sequence
  • Additions of modifications
  • Purification option

MIDLAND ensures that the yield from a synthesis is within an acceptable range. Even if a synthesis passes all the other quality control checkpoints, but does not meet our minimum expected yield, it will be resynthesized.

MALDI-TOF Mass Spectrometry

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MIDLAND was the first DNA manufacturer to use Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry as one of its quality control procedures. MIDLAND uses Delayed Extraction MALDI-TOF mass analysis to verify the molecular weight of synthetic oligonucleotides. A printout of the mass spectrum is provided for a fee of $5.00 per oligo and must be requested when the order is placed. For sequences which are purified or contain a modified base, we provide a copy of the mass spectrum at no additional charge.

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